pksM [2020-04-28 11:05:52]

polyketide synthase

Locus: BSU_17200

Isoelectric point: 5.31

Molecular weight: 475.31 kDa

Protein length: 4262 aa

Gene length: 12789 bp

Function: polyketide synthesis

Product: polyketide synthase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds

Gene

Coordinates: 1,821,553 1,834,341

The protein

Domains

• 4 Carrier domains (aa 293-367, aa 2188-2261, aa 3409-3486, aa 4135-4212) (according to UniProt)

Expression and Regulation

Operons

• Genes: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • CodY: activation, PubMed, in CodY regulon

  Regulation

  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed

  Additional information

  • this is a very large operon comprising about 75 kb

• Genes: pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
  Description: PubMed

Biological materials

Mutant

• BKE17200 (pksM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGTTATCATTTTCCCACTCC, downstream forward: _UP4_TAAAGAAATTCTCGATGCCT
• BKK17200 (pksM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGTTATCATTTTCCCACTCC, downstream forward: _UP4_TAAAGAAATTCTCGATGCCT

References

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